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SPECIES: hamster, Chinese
ECACC catalog code: 89030317
Description: Hamster Chinese ovary
Viruses: See Properties
Depositor: Dr Pohjanpelto, Deparmentt of Virology, University of Helsinki, FINLAND (Originator)
No restrictions. Patent: None Specified By Depositor
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + Ham's F12 (1:1) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 0.1% BSA. Split confluent cultures 1:5 to 1:10 i.e. seeding at 5x1,000 to 1x10,000 cells/cm2 using 0.1% cold PBS and 0.25% cold trypsin. Place flasks at 4C for 5-10 min. or until cells detach; 5% CO2; 37C. Pre-heat culture flasks in porcine gelatin (Sigma G-2625) overnight at room temperature, or for at least 2hr at 37C. Serum is only used to initially resuscitate from frozen and should be added (1%) prior to cryopreservation.
Chinese hamster ovary cell line which lacks ornithine decarboxylase activity.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Mol Cell Biol 1985;5:1385 - PMID: 4033657
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.