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TUMOR: leukemia, T cell
DSMZ catalog code: ACC 34
Morphology: single, polymorph cells in suspension
Description: human T cell leukemia established from the peripheral blood of a 7-year-old boy with acute lymphoblastic leukemia (ALL) confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. Jun Minowada, Fujisaki Cell Center, Okayama, Japan
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.3-0.9 x 106 cells/ml; optimal split ratio 1:3 to 1:5 at 37C with 5% CO2 maximum density at about 1.2 x 106 cells/ml;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (hypotetraploid (flat-moded) karyotype with 1.8% polyploidy; 83<4n>XX, -Y, -Y, -2, -4, +6, +8, -9, -9, -10, -13, +20, -21, -21, -22, -22, der(8)t(8;?)(p1;?), der(14)t(14;?)(p11;?), der(19)t(19;?13)(q13;q11)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD2+ CD3+ CD4+ CD5+ CD6+ CD7+ CD8- CD13+ TCR_/beta-) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (negative for reverse transcriptase, HTLV-I/II, HIV-1)
- Acta Haematol Jpn 1978;41:140
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.