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DSMZ catalog code: ACC 201
Morphology: adherent epithelial cells growing as confluent monolayers
Description: human gastric adenocarcinoma established from the primary tumor of a 72-year-old man with gastric adenocarcinoma in 1987 confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. H. P. Vollmers, Institute for Pathology, Wuerzburg, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS split confluent culture 1:8 every 5-6 days using trypsin/EDTA; seed out with 1-2 x 106 /25 cm 2 flask; initially after thawing, cells may grow slowly and loosely adherent; at 37C with 5% CO2 cell harvest of about 0.3 x 106 cells/cm 2;
doubling time of about 50 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 106 cells/ampoule;
contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (human karyotype) * DNA fingerprinting (unique DNA profile with (gtg)5 multilocus probe) * immunological analysis (cytokeratin+, desmin-, endothel-, GFAP-, neurofilament-, vimentin-) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase negative)
- Virchows Arch B Cell Pathol 1993;63:335-343
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.