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SPECIES: mouse, DBA/2
DSMZ catalog code: ACC 1
Morphology: single, round cells in suspension; some cells may be adherent
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Description: mouse mastocytoma established from the mastocytoma tumor of a DBA/2 mouse treated with methylcolanthrene; used as target cells for cytotoxic T cell assays; as reported cells exhibit no effector activity in an antibody-dependent cell mediated cytotoxic system confirmed as mouse with IEF of LDH, NP, PEP B
Viruses: ELISA. reverse transcriptase negative
Depositor: Dr. H. G. Drexler, DSMZ, Braunschweig, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.5-5 x 105 cells/ml, cells grow very fast; split 1:5 to 1:10 every 3-4 days at 37C with 5% CO2 maximum cell density at > 1 x 106 cells/ml;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (near-diploid karyotype; 41(36-43)<2n>; 1-2 centric fusion marker present) * isoenzymes (confirmed as human with IEF of LDH, NP, PEP B)
Availability in cell line catalogues: ATCC: TIB 64
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- Cancer Res 1977;37:546-550 - PMID: 318922
- Cell Immunol 1973;9:60-66 - PMID: 4270287
- J Exp Med 1976;143:1528-1533 - PMID: 1083890
- J Immunol 1973;111:389-394 - PMID: 4123976
- J Immunol 1977;119:950-954 - PMID: 894031
- J Natl Cancer Inst 1957;18:587
- Nature 1974;249:49-51 - PMID: 4208429
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.