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SPECIES: mouse, NIH Swiss
DSMZ catalog code: ACC 253
Morphology: cells grow as adherent fibroblastic monolayers
Description: NIH-3T3 (Swiss mouse embryo) derivative-retroviral packaging cell line derived from NIH-3T3 cells; high titer ecotropic helper-free packaging cell line; generated with separate gagpol and ecotropic env expression constructs with minimal sequence overlap and decreased sequence homology achieved by 'codon wobbling'; prior to use in nude mice, cells need to be subcloned; classified as risk category S1 according to the German Central Commission for Biological Safety (ZKBS) confirmed as mouse with IEF of MDH, NP
Viruses: ELISA: reverse transcriptase positive
Depositor: Dr. W. Dirks, DSMZ, Braunschweig, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Dulbecco's MEM + 10% NCS split confluent culture 1:5 every 3-4 days using trypsin/EDTA at 37C with 5% CO2 cell harvest of about 5-8 x 106 cells/80 cm 2;
doubling time of about 20-30 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2-3 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (mouse karyotype) * isoenzymes (confirmed as mouse with IEF of MDH, NP) * reverse transcriptase (reverse transcriptase not detected)
- Nucleic Acids Res 1990;18:3587-3596 - PMID: 2194165
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.