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SPECIES: human, Caucasian
TUMOR: carcinoma, squamous cell
ECACC catalog code: 96061336
Description: Human Caucasian oesophageal squamous cell carcinoma
Viruses: See Properties
Depositor: Drs J C Rockett/A Morriss, Department of Biological Sciences, University of Warwick and Dr S J Darnton, Birmingham Heartlands Hospital, UK (Originator)
Restricted. Patent: None Specified By Depositor
Properties: Receptors: MHC class II Applications: Study of oesophageal cancer
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:8 i.e. seeding at 1x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
The cell line OE47, also known as JROECL47, was established in 1994 from a squamous carcinoma with focal adenocarcinoma of mid oesophagus of a 76 year old male patient. The tumour was identified as pathological stage IIA (UICC) and showed poor differentiation. The cell line OE47 expresses HLA-A,-B and -C (MHC class I) constitutively. Expression of HLA-DR (MHC class II) and ICAM-1 can be induced by treatment with interferon-gamma. OE47 is nearly diploid with loss of chromosome 17 and frequent double minutes. The cells express epithelial cytokeratins and are tumourigenic in nude mice. OE47 and OE50 have been demonstrated, by DNA fingerprint analysis at the ECACC, to represent cell lines from the same individual. However, these cell lines have different morphology and differentiation patterns. The original tumour of origin was a carcinoma of mixed cell type: exhibiting a focal glandular pattern consistent with differentiation towards adenocarcinoma. OE50 is more pleomorphic than OE47 which does not stain by D/PAS (consistent with its squamous origin). OE50 is D/PAS positive. The two cell lines probably represent independent sub-lines of a mixed tumour which show karyological differences (Dr. A. Morris personal communication).
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Br J Cancer 1997;75:258 - PMID: 9010035
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.