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TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, acute myeloid
DSMZ catalog code: ACC 99
Morphology: single, round to oval cells in suspension
Description: human acute myeloid leukemia established from the peripheral blood of a 65-year-old man with acute myeloid leukemia (AML FAB M4) in 1986 at diagnosis; cells were described to express the retinoic acid receptor gene confirmed as human with IEF of MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HIV-, HTLV-I/II-
Depositor: Dr. E. A. McCulloch, Ontario Cancer Institute, Toronto, Canada
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80-90% alpha-MEM + 10-20% FBS cells are difficult to culture! cells grow slowly; maintain at 0.5-1.5 x 106 cells/ml at 37C with 5% CO2 maximal density at about 4 x 106 cells/ml;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (hyperdiploid karyotype with 3.8% polyploidy;48(43-49)<2n>XY,+6,+8,der(1)inv(1)(p36q31)t(1;6)(q13;p12), der(2)t(2;17)(p23;q24.1)del(2)(q14.2q36),der(3)t(1;3)(p36;p25),ins(3;2)(q21;q14.2q36),t(5;8)(q11.2;q24),der(6)t(1;6)(q31;p12 t(3;6)) * cytogenetics 2 ((q26;q24),inv(12)(p13.3q13.2),t(13;14)(q32/33;q24.2),der(17)t(2;17)(p23;q24.1);sdl with +der(5);carries apparent variant translocations involving several ANLL breakpoints: 1p36,3q21,3q26 (megakaryocytic abnormalities),12p13) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD13+ CD14+ CD15+ CD19- CD33+) * isoenzymes (confirmed as human with IEF of MDH, NP) * reverse transcriptase (negative for reverse transcriptase, HTLV-I/II, HIV-1)
Availability in cell line catalogues: ICLC: HTL01010
- Leukemia 1989;3:264-269 - PMID: 2538684
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.