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SPECIES: mouse, C3H/Law
DSMZ catalog code: ACC 226
Morphology: large and flat regular arranged cells, growing in up to 3 cell layers
Description: mouse urinary bladder established from an adult female C3H/Law mouse; non-tumorigenic in vitro immortalized urine bladder cell line with aberrant growth factor response, aneuploid (chromosome number 70-75) confirmed as mouse with IEF of AST, NP
Viruses: ELISA: reverse transcriptase negative
Depositor: Dr. W. I. de Boer, Erasmus University, Rotterdam, The Netherlands
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
45% Ham's F10 + 45% Dulbecco's MEM + 10% FBS + 5 æg/ml bovine insulin + 5 æg/ml human transferrin + 50 nM hydrocortisone + 5 ng/ml selenite + 10 æM HEPES split confluent culture 1:4 every 6-7 days using trypsin/EDTA at 37C with 5% CO2 harvest of 2-3 x 106 cells/80 cm 2;
doubling time of about 60-75 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (murine hypotetraploid karyotype with 14% polyploidy and 1-3 d-min in 34% cells; 70(64-74)<4n>; 1-3 centric fusion markers present) * isoenzymes (confirmed as mouse with IEF of AST, NP) * reverse transcriptase (reverse transcriptase not detected)
- Int J Cancer 1993;54:1022-1027 - PMID: 7687588
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.