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ICLC catalog code: HTL97025
Monoclonal continuous culture, grown as monolayer
SPECIES: human, Caucasian (male, 22 years)
TUMOR: carcinoma, embryonal pluripotent
DEPOSITOR: Dr G. Angelini, Biotechnology Dept, IST at ABC, Genoa, Italy.
Cell line available for distribution, as growing culture. For non-commercial investigative use only
PROPERTIES: The cell line differentiates when exposed to retinoic acid (RA) or hexamethylene bisacetamide (HMBA).
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM (4.5 g/L glucose) + 10% FBS + 2mM L-Glutamine; Freezing medium: 90% FBS + 10% DMSO; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-5x104 cells/cm2; 37C, 5% CO2. After thawing the cells look very suffering and need some days to recover. Passage: 22, mycoplasma negative, HOECHST and PCR
Validated by: HLA-DP beta (confirmed as human by PCR) * isoenzymes (confirmed as human with NP)
- Dev Biol 1984;103:285-293
- Lab Invest 1984;50:147-162 - PMID: 6694356
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.