This is the complete description of the cell line that you selected.
By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).
TISSUE/ORGAN: kidney, fibroblast
DSMZ catalog code: ACC 172
Morphology: fibroblast-like cells with a fusiform shape
Description: rat kidney fibroblasts cell line was cloned from a mixed culture of normal rat kidney cells; cells exhibit contact inhibition and are very sensitive; indicator cells for viral or chemical transformation; this cell line is frequently used for TGF-ss bioassays; (= ATCC CRL 1570) confirmed as rat with IEF of AST, NP, PEP B
Viruses: ELISA. reverse transcriptase negative
Depositor: Dr. J. Wehland, GBF, Braunschweig, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
95% Dulbecco's MEM + 5% FBS split culture 1:4 to 1:5 about 2-3 times a week using trypsin/EDTA when the cells have reached 3/4 of confluence at 37C with 5% CO2 cell harvest of about 3.5 x 106 /80 cm 2;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (near-diploid rat karyotype with 4% polyploidy; 40(37-42)<2n>) * isoenzymes (confirmed as rat with IEF of AST, NP, PEP B)
Availability in cell line catalogues: ATCC: CRL 1570, ECACC: 86101301
- J Bacteriol 1966;92:1133-1140 - PMID: 5926742
- J Cell Physiol 1978;94:335 - PMID: 304450
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.