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SPECIES: mouse / mouse
DSMZ catalog code: ACC 205
Morphology: round cells growing singly or in small clumps in suspension, some cells are slightly adherent
Description: mouse hybridoma (anti-chicken retina) cell line was established by fusion of mouse NS-1 cells with spleen from a BALB/c mouse immunized with Triton X114-purified chicken retina E10 proteins; described as secreting a mouse monoclonal antibody (IgG) reacting with chicken retina (Mueller glia cell-specific antigen) confirmed as mouse with IEF of LDH, NP
Viruses: ELISA: reverse transcriptase positive
Depositor: Prof. B. Schlosshauer, Natural and Medical Science Institute (NMI), Reutlingen, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80-85% RPMI 1640 + 10-15% FBS + HFCS ('Hybridoma Fusion and Cloning Supplement') for the first 1-2 weeks post-thawing cells are difficult to culture! maintain at ca. 0.2-0.8 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 2-3 days; seed out at ca. 0.2 x 106 cells/ml; after thawing cultivate cells for the first 1-2 weeks in 24- or 12-well-plates using HFCS at 37C with 5% CO2 maximal density at ca. 0.8 x 106 cells/ml;
doubling time of ca. 35 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 4-5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (murine karyotype) * immunological analysis (secretion of a mouse IgG2 monoclonal antibody; we suggest to subclone the culture as about 3% of the cells reacted strongly with FITC anti-mouse IgM) * isoenzymes (confirmed as mouse with IEF of AST, MDH, PEP B) * reverse transcriptase (reverse transcriptase not detected)
- Dev Biol 1990;138:348-358 - PMID: 2318340
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.