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SPECIES: rat, Osborn-Mendel
TISSUE/ORGAN: kidney, fibroblast
ECACC catalog code: 86032002
Susceptibility to viruses: vesicular stomatitis
Description: Rat kidney fibroblast
Viruses: See Properties
Depositor: Mrs M Freshney, The Beatson Institute, Glasgow, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus titration
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Derived from normal rat kidney (Rattus norvegicus). The cells are useful for focus-forming assays in virus titrations.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
- Int J Cancer 1973;12:654 - PMID: 4134008
- J Bacteriol 1966;92:1133-1140 - PMID: 5926742
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.