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SPECIES: mouse, NFS/N
ECACC catalog code: 88041904
Description: Mouse pre-B lymphoblast
Viruses: See Properties
Depositor: Mr T Littlewood, Ludwig Institute for Cancer Research, Cambridge, UK
No restrictions. Patent: None Specified By Depositor
Properties: Products: Ecotropic and mink cell focus-forming viruses Applications: Virus studies: MuLV, ecotropic and focus-forming viruses
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 1mM Oxaloacetic acid + 0.2U/ml Bovine Insulin + 2ug/ml Transferrin + 50ÁM 2-Mercaptoethanol (2ME) + 10% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37C.
Established from a lymphoma produced in an NFS/N mouse by injecting CaS-25M ecotropic MuLV. Cells show markers of large pre-B lymphoblasts and spontaneously produce ecotropic and mink cell focus-forming viruses.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1693
- J Immunol 1982;129:751 - PMID: 6282970
- J Immunol 1984;133:744-753 - PMID: 6330201
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.