This is the complete description of the cell line that you selected.
By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).
SPECIES: mouse, C3H/HeN
ECACC catalog code: 90111915
Description: Mouse C3H/HeN connective tissue
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Growth factors and enzymatic studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
NCTC 135 + 2mM Glutamine + 10% Horse Serum (HS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C. Freeze in 9% glycerol/91% FBS.
Karyotype: Modal No. 52
From NCTC strain 1742 of NCTC clone 1328; from culture of subcutaneous areolar and adipose connective tissue taken from normal male C3H/HeN. Used in studies involving neoplastic properties, growth rates, rates of glycolysis and enzymatic activities.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 11
- Cancer Res 1958;18:747
- J Natl Cancer Inst 1954;15:215
- J Natl Cancer Inst 1958;20:121
- J Natl Cancer Inst 1960;23:1059
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.