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SPECIES: mouse, C57B1/KaLw
ECACC catalog code: 90102530
Susceptibility to viruses: polyoma virus
Description: Mouse C57B1/KaLw embryo
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Transformation and virus studies: Polyoma virus
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
NCTC 135 100% + 2mM Glutamine. Split confluent cultures 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using mechanical methods to dislodge the cells (e.g. scraping); 5% CO2; 37C.
Karyotype: Modal No. 71-73; hypotetraploid
Derived by cloning from a parent line produced from normal mouse embryo tissue (C57B1/KaLw). The cells have spontaneously transformed in vitro. The cells are negative for polyoma virus infection but are susceptible to the virus.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 63
- Science 1967;156:85 - PMID: 4289746
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.