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SPECIES: mouse, DBA/2
TUMOR: lymphoid neoplasm
ECACC catalog code: 90111912
Morphology: Fibroblast-like with some round cells
Description: Mouse lymphoid
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Interleukin 1 (IL-1) Applications: Nutritional and chromosome studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
NCTC 135 100% + 2mM glutamine. No serum is required. Split confluent cultures 1:2. Remove one-half of the old medium, add fresh, dislodge cells by scraping, aspirate and dispense suspension into new flasks. 5% CO2; 37C. NB: freeze in 8-10% glycerol and 90-92% Growth medium
Karyotype: 2n = 40
A chemically defined medium derivative of P338.D1. Produces IL-1 in response to PMA and LPS. Supports polyoma virus in serial transfer.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 46.1
- Exp Cell Res 1963;32:212
- J Immunol 1978;120:1497 - PMID: 149171
- Proc Soc Exp Biol Med 1959;100:229
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.