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ICLC catalog code: HTL97001
Monoclonal continuous culture, grown as monolayer
SPECIES: human, Caucasian (female, 65 years old)
TUMOR: carcinoma, non small cell
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. Any commercial use must be negotiated with the University of Texas
PROPERTIES: The best differentiated available bronchial carcinoid cell line. The cells express easily detectable levels of p53 mRNA, and are not able to synthesise gastrin releasing peptide (GRP); neuromedin B peptide synthesis (NMB).
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4 x104 cells/cm2; 37C, 5% CO2. Passage: 60, mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmes as human with AST, G6PD, NP)
Availability in cell line catalogues: ECACC: 94060303
- Cancer Res 1992;52 (Suppl):2732-2736
- J Clin Invest 1987;79:1629-1634 - PMID: 3034978
- Science 1989;246:491-494 - PMID: 2554494
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.