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SPECIES: human, Caucasian
TISSUE/ORGAN: lymphoblastoid B cell
DSMZ catalog code: ACC 120
Morphology: single, polymorph cells or small clumps in suspension
TRANSFORMED BY: Epstein-Barr
Description: human B-lymphoblastoid cells established from peripheral blood lymphocytes of a 27-year-old white Caucasian woman by Epstein-Barr virus (EBV) transformation in 1990; suitable normal control cell line for toxicity or radiosensitivity assays confirmed as human with IEF of AST, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV+, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. R. A. F. MacLeod, DSMZ, Braunschweig, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain; seed out at ca. 0.5 x 106 cells/ml at 37C with 5% CO2 maximal density at about 1.5 x 106 cells/ml;
doubling time of ca. 50-60 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (near diploid with hypodiploid sideline; 45/46(42-46)<2n>XX/X,-X; no structural rearrangements detected) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD10- CD13- CD19+ CD20+ CD37+ HLA-DR+ cy/smIgG+ cy/smIgM- cy/sm+ cy/sm-) * isoenzymes (confirmed as human with IEF of AST, NP) * reverse transcriptase (reverse transcriptase negative)
- Mutagenesis 1992;7:285-290 - PMID: 1518412
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.