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TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, chronic myeloid
DSMZ catalog code: ACC 131
Morphology: small, single, round cells in suspension
Description: human chronic myeloid leukemia in blast crisis established from the peripheral blood of a 3-year-old girl with chronic myeloid leukemia (CML) in blast crisis in 1975; cells were described to be TdT-positive and to carry the Philadelphia-chromosome t(9;22) confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. J. Minowada, Fujisaki Cell Center, Okayama, Japan
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80-90% RPMI 1640 + 10-20% FBS cells are difficult to culture! maintain at 1.0-2.0 x 106 cells/ml; seed out at ca. 2 x 106 cells/ml; cells grow initially slowly; it might be of benefit to start culture in 24-well plates at 37 C with 5% CO2 maximal density at about 3-5 x 106 cells/ml;
doubling time of ca. 48 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hypodiploid karyotype with 5% polyploidy; 45(42-47)<2n>X, -X, der(9)t(9;22)(q24;q12)x1-2, dup(13)(q21), der(22)t(9;22)(q34;q12)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD10+ CD13- CD19+ CD37+ HLA-DR+ cyIgG- cyIgM+) * isoenzymes (confirmed as human with IEF of AST, MDH, NP)
- J Natl Cancer Inst 1977;59:83-87 - PMID: 301573
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.