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SPECIES: mouse, A/Jax
DSMZ catalog code: ACC 103
Morphology: round, single, slightly adherent cells, occasionally with short processes
Description: mouse neuroblastoma 6-thioguanine resistant mutant of cell line N18 that was established from a mouse of strain A/Jax with neuroblastoma C1300; cells were described to form cAMP in response to prostaglandin E1 and to be aneuploid (modal chromosome number of 81) confirmed as mouse with IEF of AST, MDH, NP, PEP B
Viruses: ELISA: reverse transcriptase positive
Depositor: Dr. B. Hamprecht, Physiologisch-Chemisches Institut, Tuebingen, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Dulbecco's MEM + 10% FBS + 100 śM 6-thioguanine split culture 1:5 to 1:10 every 3-5 days (cells grow adherent, but can be detached without trypsin) at 37 C with 5-10% CO2 about 1.5 x 107 cells/80 cm 2;
doubling time of ca. 40 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: isoenzymes (confirmed as mouse with IEF of AST, MDH, NP, PEP B)
- Int Rev Cytol 1977;49:99-170 - PMID: 16829
- Nature New Biol 1972;235:225-231 - PMID: 4111970
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.