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TUMOR: leukemia, acute monocytic
DSMZ catalog code: ACC 102
Morphology: single, round cells growing in suspension
Description: human acute monocytic leukemia established from a 10-year-old boy with acute monocytic leukemia (AML FAB M5) at diagnosis; cells were described to carry a 4;11 translocation; patented cell line confirmed as human with IEF of MDH, NP, PEP B
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. H. G. Drexler, DSMZ, Braunschweig, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.2-1.0 x 106 cells/ml; split carefully; do not exceed a cell density of about 1 x 106 cells/ml; cells are difficult to culture and grow slowly at first; addition of recombinant GM-CSF or IL-3 (or as 10-20% vol conditioned medium of cell line 5637, DSM ACC 35) or increasing the FBS content to 20% can be of advantage; cultivation in 24-well-plates appears to be better than in large culture flasks at 37C with 5% CO2 saturation density at about 1.0 x 106 cells/ml;
doubling time of ca. 50 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hyperdiploid; 48(46-48)<2n>XY, +8, +18, +19, -21, t(4;11)(q21;q23)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD4+ CD5- CD8- CD10- CD13+ CD14- CD15+ CD19- CD21- CD25- CD33+ CD37- DR+) * isoenzymes (confirmed as human with IEF of MDH, NP, PEP B) * reverse transcriptase (negative for HTLV-I/II, HIV-1)
Availability in cell line catalogues: ATCC: CRL 9591
- Blood 1987;70:192 - PMID: 3496132
- J Immunol 1987;139:3348-3354 - PMID: 3500218
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.