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TISSUE/ORGAN: nervous system
TUMOR: nervous tissue glial tumor
ECACC catalog code: 85042304
Morphology: Glial / neuronal
Transformed by: Chemical Agent Back To Chemical Agents' WebPage
Description: Rat nervous tissue glial
Viruses: See Properties
Depositor: Dr D Schubert, Salk Institute, San Diego, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Neurobiological studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Ethyl nitrosourea induced tumour cell line. Neuronal and glial cells useful in research of distribution of neurotransmitter synthesis and brain specific antigens among nerve and glia. Morphology significantly affected when grown in serum free media.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Nature 1974;249:224 - PMID: 4151463
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.