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SPECIES: mouse / mouse
DSMZ catalog code: ACC 211
Morphology: round cells growing in suspension
Description: mouse hybridoma cells are a subclone derived from B13.29 (first described as clone 9.9) by fusion of BALB/c spleen cells with SP2/0-AG14 myeloma cells; this hybridoma is a non-producer; cells are IL6-dependent confirmed as mouse with IEF of AST, PEP B
Viruses: ELISA: reverse transcriptase negative
Depositor: Dr. L. Aarden, Netherlands Red Cross Blood Transfusion Service, Amsterdam, The Netherlands
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS + 50 śM mercaptoethanol + 50 pg/ml human IL-6 split saturated culture 1:3 to 1:4 every 2-3 days; seed out at ca. 0.5 x 106 cells/ml, best in 24- or 6-well plate at 37C with 5% CO2 maximal density at about 0.8 x 106 cells/ml;
doubling time of ca. 30-40 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (apparently murine chromosome morphology with extensive centric fusions) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ICLC: HYL98004, ECACC: 96080128
- Eur J Immunol 1987;17:1411-1416 - PMID: 3500054
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.