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ECACC catalog code: 85042501
TRANSFORMED BY: SV40
Description: Human fetal lung, SV40 transformed
Viruses: See Properties
Depositor: Dr R Cox, MRC Radiobiology Unit, Chilton, Didcot, UK
No restrictions. Patent: None Specified By Depositor
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Karyotype: Modal No. = 60-80 hyperdiploid
A clonal derivative of an SV40 transformed permanent line of MRC-5. Selection was made using X-ray induction in the presence of 2µg/ml thioguanine. The cells are HAT sensitive.
Research council deposit: Yes;
Price Code: B;
Validated by: isoenzymes (species confirmation)
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.