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SPECIES: mouse, C3H/An
TISSUE/ORGAN: areolar and adipose
ECACC catalog code: 85011408
Description: Mouse C3H/An sc areola and adipose tissue
Viruses: See Properties
Depositor: Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Chromosome analysis and hybridization studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Karyotype: 2n = 51
Derivative of NCTC clone 929 obtained by progressive selection in BUdR and has a cloning efficiency of 70-80%.
Research council deposit: Yes;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Exp Cell Res 1966;41:190 - PMID: 4952025
- J Cell Sci 1969;5:93-120 - PMID: 4311079
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.