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ECACC catalog code: 90110707
Description: Mouse BALB/c plasmacytoma, IgG secreting
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Immunoglobulin G (IgG1) Applications: Tumourigenicity studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
L-15 + 2mM Glutamine + 20% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x100,000 cells/ml; 37C. The cells have a maximum density of 1-2x1,000,000 cells/ml. CO2 is not required.
Karyotype: Modal No. 43, 81-85 hyperdiploid
The original plasma cell tumour was induced by intraperitoneal injections of mineral oil in highly inbred BALB/CJ mice. The line secretes IgG1, is hyperdiploid and produces a balanced synthesis of monomeric 4-chain gamma F/immunoglobulin.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 130
- J Mol Biol 1964;9:537
- J Natl Cancer Inst 1960;25:847
- J Natl Cancer Inst 1968;41:1083 - PMID: 4176942
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.