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ECACC catalog code: 85022106
Description: Mouse myeloma
Viruses: See Properties
Depositor: Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Products: Immunoglobulin A kappa
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10-20% Fetal Bovine Serum (FBS). Maintain cultures between 1-2x1,00,000 cells/ml; 5% CO2; 37C. Do not allow cell counts to rise above 1,000,000 cells/ml as rapid death occurs at higher concentrations.
Cells are killed by low concentrations (10nM) of dexamethasone. Established in vitro from the MOPC 315 plasmacytoma induced by mineral oil injections in a Balb/c mouse. Secretes an IgA kappa antibody which recognizes DNP-TNP substituted proteins.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: TIB 23
- J Immunol 1972;109:674 - PMID: 5074326
- J Natl Cancer Inst 1971;43:403 - PMID: 5000540
- Physiol Rev 1972;52:631 - PMID: 4555516
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.