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TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, acute monocytic
DSMZ catalog code: ACC 252
Morphology: single, round/multiformed cells in suspension
Description: human acute monocytic leukemia established from the peripheral blood of a 64-year-old man with acute monocytic leukemia (AML FAB M5) at relapse in 1985 following myeloid metaplasia; sister cell line of MONO-MAC-6 (DSM ACC 124) confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. H. W. L. Ziegler-Heitbrock, Institute of Immunology, Munich, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS + 2 mM L-glutamine + 1x non-essential amino acids + 1 mM sodium pyruvate maintain at 0.3-1.0 x 106 cells/ml; split ratio 1:2 to 1:3 every 2-3 days; seed out at ca. 1 x 106 cells/ml at 37C with 5% CO2 maximal density at 1.0-1.5 x 106 cells/ml;
doubling time between 30 and 50 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (human near-diploid karyotype with 10% polyploidy; 46/47(43- 52)<2n>X/XY, +8, +13, +21, DM, add(3)(q27), t(9;11)(p13;q23), t(10;13)(q23.1;q13), t(12;17)(q21;q11), ?t(21;21)(q22.1;q11/21; carries 9;11 translocation (AML-M5); diploi version) * cytogenetics 2 (of its sister MONO-MAC-6 with fewer in vitro rearrangements) * immunological analysis (CD3- CD13+ (95%) CD14(+) (3%) CD15+ (99%) CD19- CD33- HLA-DR+ (50%) CD68+) * isoenzymes (confirmed as human with IEF of AST, MDH)
- Int J Cancer 1988;41:456-461 - PMID: 3162233
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.