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TISSUE/ORGAN: nervous system
TUMOR: nervous tissue neuronal tumor
ECACC catalog code: 85042305
Transformed by: Chemical Agent Back To Chemical Agents' WebPage
Description: Rat nervous tissue neuronal
Viruses: See Properties
Depositor: Dr D Schubert, Salk Institute, San Diego, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Neurobiological studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Ethyl nitrosourea induced tumour cell line. Neuronal cells useful in research of distribution of neurotransmitter synthesis and brain specific antigens among nerve and glia. Cell morphology significantly affected when grown in serum free media.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Nature 1974;249:224 - PMID: 4151463
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.