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SPECIES: human, Caucasian
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, hairy cell
ECACC catalog code: 90021503
TRANSFORMED BY: Epstein-Barr
Endogenous viruses: contains Epstein-Barr * contains HTLV-2
Description: Human Caucasian peripheral blood hairy cell leukaemia
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Epstein Barr Virus (EBV), Epstein-Barr viral capsid antigen (VCA), Epstein Barr Nuclear Antigen ( EBNA), EBV genome and membrane Immunoglobulin G (IgG) Applications: Tumourigenicity and virus studies: EBV
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
IDMEM + 2mM glutamine + 20% FBS Maintain cultures between 3x100,000 to 1x1,000,000 cells/ml; 5% CO2; 37C
Karyotype: 2n = 46, diploid
An EBV-transformed B-lymphoblast from a 33 year male Caucasian. Cells produce EBV VCA and EBNA as well as EBV genome and membrane IgG. The line is infected with HTLV-2 and must be handled under laboratory containment level 3.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 245
- Ann Intern Med 1978;88:323 - PMID: 305224
- Nature 1983;305:502 - PMID: 6312323
- Proc Natl Acad Sci U S A 1983;80:7006 - PMID: 6316341
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.