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SPECIES: human, Caucasian
TUMOR: sarcoma, osteogenic
ECACC catalog code: 87070201
Transformed by: Chemical Agent Back To Chemical Agents' WebPage
Description: Human Caucasian osteosarcoma, chemically transformed
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Transformation and tumourigenicity studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:4 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Karyotype: Modal No. 70, 71; hypertriploid
A chemically transformed cell line (MNNG 0.01ug/ml) derived from an osteosarcoma of a 13 year female Caucasian. It grows to high saturation density and produces tumours in nude mice.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1547
- Int J Cancer 1977;19:505 - PMID: 265298
- Nature 1975;256:751 - PMID: 1057046
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.