This is the complete description of the cell line that you selected.
By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, B cell
DSMZ catalog code: ACC 138
Morphology: single, round to polygonal cells in suspension
Description: human B cell leukemia established from the peripheral blood of a 20-year-old man with acute B cell leukemia (ALL FAB L3) in partial remission in 1981 confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. G. Roos, University of Umea, Umea, Sweden
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Ham's F10 + 10% FBS cells are difficult to culture! maintain at 0.2-0.5 x 10 6 cells/ml; cells grow very slowly; split carefully at 37C with 5% CO2 maximal density at 0.75 x 106 cells/ml;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x10 6 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (near diploid; 46(45-47)<2n>XY, dup(1)(q21q41), del(6)(q21), t(8;14)(q24;q32), i(13q)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD10+ CD13- CD19+ CD37+ HLA-DR+ smIgG- smIgM+) * isoenzymes (confirmed as human with IEF of AST, MDH, NP)
- Leuk Res 1982;6:685-693 - PMID: 6296550
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.