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TUMOR: lymphoma, B cell
DSMZ catalog code: ACC 48
Morphology: lymphoblast-like cells growing in clumps or as single cells in suspension
Description: human B cell lymphoma established in 1996 from the diagnostic pre-treatment pleural effusion sampled shortly before death of a 49-year-old HIV+ homosexual male with primary effusion lymphoma (PEL) and previous history of Kaposi's sarcoma; autopsy revealed involvement of serous membranes without formation of solid tumor mass; cells were described to be EBV+, HHV-8+ and to carry a BCL-6 point mutation confirmed as human with IEF of AST, LDH, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV+, HBV-, HCV-, HHV-8+, HIV-, HTLV-I/II-
Depositor: Prof. A. Carbone, Centro di Riferimento Oncologico, Aviano, Italy
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80% RPMI 1640 + 20% FBS split saturated culture about 1:2 to 1:3 every 2-4 days; maintain at ca. 0.2-0.5 x 106 cells/ml feeding cultures daily; seed out after thawing at ca. 0.5 x 106 cells/ml at 37C with 5% CO2 maximal density at ca. 1.5 x 106 cells/ml;
doubling time of ca. 20-40 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hypotetraploid; 89(84-96)<4n>XX, -X, -X, -2, -4, -5, -16, +3m, t(5;6)(q13;q21-23), inv(7)(q31q35), del(7)(q22), del(9)(p12), inv(9)(p13q13), t(10;14)(q26;q11.2), del(12)(q21), ins(13)(q34q22); 10;14 translocation appears to be va iant) * cytogenetics 2 (of more usual translocation with a breakpoint at 10q24) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD2- CD3+ CD4+ CD5+ CD6+ CD7+ CD8- CD13- CD19-) * isoenzymes (confirmed as human with IEF of AST, MDH, NP) * reverse transcriptase (negative for reverse transcriptase, HTLV-I/II, HIV-1)
- Human Cell 1988;1:263-274 - PMID: 2856501
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.