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TUMOR: malignant melanoma
ECACC catalog code: 93082609
Morphology: Fibroblast (granular)
Endogenous viruses: contains varicella zoster
Susceptibility to viruses: varicella zoster
Description: Human malignant melanoma
Viruses: See Properties
Depositor: Dr D R Harper, Department of Virology, St Bartholomews Hospital, London, UK
No restrictions. Patent: None Specified By Depositor
Properties: Products: Melanin Applications: Virus studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA (0.5% trypsin in 0.53mM EDTA in modified Puck's saline A); 5% CO2; 37C.
Derived from a human melanoma by Prof. C Grose (1978). The cells support growth of varicella-zoster virus (VZV) isolates at 36C although viral growth is optimal at 32C.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ICLC: HTL97019
- Infect Immun 1978;19:199-203 - PMID: 203532
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.