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DSMZ catalog code: ACC 74
Morphology: epithelial-like adherent cells growing in monolayers
Description: human melanoma established from the primary tumor of a 58-year-old woman with melanoma in 1977 confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. H. W. L. Ziegler-Heitbrock, Institute of Immunology, University of Munich, Munich, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS split confluent culture 1:5 every 3-4 days using trypsin/EDTA; seed out at ca. 3 x 106 cells/80 cm 2 at 37C with 5% CO2 about 10 x 106 cells/80 cm 2 flask;
doubling time of ca. 28 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (cytokeratin- desmin- endothel- GFAP- neurofilament-vimentin+) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase negative)
- Cancer Res 1985;45:1344-1350 - PMID: 3971378
- Eur J Immunol 1981;11:825-831 - PMID: 6895502
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.