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SPECIES: dog, cocker spaniel
ECACC catalog code: 84121903
Susceptibility to viruses: adenovirus 4 * adenovirus 5 * canine distemper virus * canine hepatitis * Coxsackie B * influenza A * influenza B * reovirus * reovirus 2 * reovirus 3 * swine fever virus * swine vesicular disease virus * vaccinia * vesicular stomatitis
Description: Canine Cocker Spaniel kidney
Viruses: See Properties
Depositor: Dr D Tyrrell, MRC Common Cold Unit, Salisbury, UK (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: SVEV, VSV, Vaccinia, adeno and reoviruses, Coxsackie B5 et al
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C. Cells attach firmly and require at least 2 PBS washes prior to addition of trypsin/EDTA.
Karyotype: 2n = 78
From kidney of normal female adult Cocker Spaniel in 1958 by SH Madin and NB Darby (Madin Darby Canine Kidney). Supports growth of wide range of animal viruses: VSV (Indiana strain), infectious Canine Hepatitis, Vaccinia, Coxsackie B5, Adeno and reo viruses, SVEV.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 34, IZSBS: BS CL 64
- Am J Vet Res 1968;29:1033 - PMID: 4967124
- Appl Microbiol 1968;16:588 - PMID: 5647517
- Proc Soc Exp Biol Med 1958;98:574
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.