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DSMZ catalog code: ACC 174
Morphology: adherent, epithelial-like cells
Susceptibility to viruses: alpha viruses * bovine diarrhea virus * infectious bovine rhinotracheitis * parainfluenza 3 * vesicular stomatitis
Description: bovine kidney cells cells were derived from a kidney of a normal adult steer in 1957; cells are BVD-free (bovine diarrhea virus) confirmed as bovine with IEF of AST, G6PD, NP
Viruses: ELISA. reverse transcriptase negative
Depositor: Dr. R. Riebe, Friedrich-Loeffler-Institut, BFA, Insel Riems, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
95% Dulbecco's MEM + 5% FBS optimal split ratio at 1:4 to 1:6 using trypsin/EDTA; cells grow within 2-3 days to confluence; seed out 1-2 x 106 cells/80 cm 2 at 37C with 5% CO2 cell harvest of about 5-10 x 106 cells/80 cm 2;
doubling time of ca. 35 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: isoenzymes (confirmed as bovine with IEF of AST, G6PD, NP)
Availability in cell line catalogues: ATCC: CRL 6071, IZSBS: BS CL63, ECACC: 90050801
- Proc Soc Exp Biol Med 1958;98:574
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.