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SPECIES: human, Caucasian
DSMZ catalog code: ACC 115
Morphology: epithelial-like cells growing as monolayers
Endogenous viruses: contains type B * contains type C
Description: human breast adenocarcinoma established from the pleural effusion of a 69-year-old Caucasian woman with metastatic mammary carcinoma (after radio- and hormone therapy) in 1970; cells were described of being positive for cytoplasmic estrogen receptors and having the capability to form domes confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: obtained from ATCC (HTB 22), Rockville, Maryland, USA
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS + MEM non-essential amino acids + 1 mM sodium pyruvate + 10 ęg/ml bovine insulin split confluent culture 1:2 to 1:4 every 2-6 days using trypsin/EDTA; seed out at ca. 0.1-0.2 x 105 cells/cm 2 at 37C with 5% CO2 about 20-25 x 106 cells/175 cm 2;
doubling time of ca. 50 hours (range 30-72 hours);
frozen with 70% medium, 20% FBS, 10% DMSO at about 1-3 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (cytokeratin+ (100%) desmin- endothel- GFAP- neurofilament- vimentin-) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ATCC: HTB 22
- J Natl Cancer Inst 1973;51:1409-1416 - PMID: 4357757
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.