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SPECIES: human, Caucasian
ECACC catalog code: 86012803
Endogenous viruses: contains type B * contains type C
Description: Human Caucasian breast adenocarcinoma
Viruses: See Properties
Depositor: Dr M Shaw, ICI Pharmaceuticals Ltd, UK
No restrictions. Patent: None Specified By Depositor
Properties: Receptors: Reported to express the oestrogen and progesterone receptor Applications: Tumourigenicity and virus studies: Type B and C viruses
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Karyotype: 2n = 46, hypertriploid to hypotetraploid
Established from pleural effusion from 69 year female Caucasian. Cells exhibit some features of differentiated mammary epithelium including oestradiol synthesis and formation of 'domes'. Cells may carry B or C type retrovirus and is considered to represent a category 2 pathogen (P2 containment). Cells express both the wildtype and variant oestrogen receptors as well as progesterone receptor. The cells should be handled under laboratory containment level 2.
Research council deposit: No;
Price Code: A;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: HTB 22, ICLC: HTL95021
- J Natl Cancer Inst 1973;51:1409-1416 - PMID: 4357757
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.