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SPECIES: Mamestra brassicae (cabbage moth)
DSMZ catalog code: ACC 122
Morphology: polymorphic round cells, partly adherent
Description: insect - cabbage moth (Mamestra brassicae) established from the larvae of the butterfly Mamestra brassicae (family Noctuidae, subfamily Noctuinae) (Kohleule) confirmed to be not of murine or primate origin with IEF of AST, PEP B
Depositor: Dr. H. U. Leistner, Institut fuer Pathogendiagnostik, Aschersleben, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Grace's Insect (Modified) Medium + 10% FBS cells are difficult to culture! split cultures 1:2 every 2-3 days (cells grow partly adherent, but trypsin is not necessary); maintain at room temperature (about 20C) without CO2 exposure (keep lid of flask closed); confluent cultures can be stored at 4C for up to one week without loss of viability; after thawing cells might have a lag phase of up to 4 weeks at 20C (room temperature) without CO2 (keep lid of flask closed) about 5-7 x 106 cells/80 cm 2;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: isoenzymes (confirmed to be not of murine or primate origin with IEF of AST, PEP B)
- Arch Phytopathol Pflantzenschutz 1989;25:387-401
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.