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TISSUE/ORGAN: lymphoblastoid B cell
DSMZ catalog code: ACC 185
Morphology: lymphoblastoid-like cells growing in suspension singly or in clumps
TRANSFORMED BY: Epstein-Barr
Description: human B-lymphoblastoid cells (from patient with melanoma) this lymphoblastoid cell line was established by EBV transformation of peripheral blood lymphocytes; cell line was submitted as autologous to cell line MEL-HO (DSM ACC 62) that was derived from a woman with primary melanoma in 1976; however, cytogenetic and DNA fingerprinting analysis showed clearly that LCL-HO and MEL-HO are not related confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV+, HBV-, HCV-, HIV-, HTLV-I/II-
Depositor: Dr. H. W. L. Ziegler-Heitbrock, University of Munich, Institute of Immunology, Munich, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.5-1.0 x 106 cells/ml; optimal split ratio of 1:2 to 1:3 every 2-4 days at 37C with 5% CO2 saturation density at about 2.0-2.5 x 106 cells/ml;
doubling time of ca. 30 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (human diploid karyotype with near triploid side line (4%) and 2% polyploidy; 46(45-47)<2n>XX, der(12)t(12;?)(q24;?); additional pseudodiploid subclones are present with other structural and numerical rearrangements) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD13(+) CD19+ CD20+ CD37+ HLA-DR+ smIgG+ smIgM- smkappa- smlambda- cyIgG+(50%) cyIgM+(50%) cykappa+(50%) cylambda+(50%)) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase negative)
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.