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SPECIES: rat, Fisher
TISSUE/ORGAN: testis, Leydig cells
TUMOR: testicular tumor
ECACC catalog code: 89031604
Susceptibility to viruses: herpes simplex * vaccinia * vesic. stomatitis (Indiana)
Description: Rat Fischer Leydig cell testicular tumour
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C.
Karyotype: 2n = 42
A cell line from a spontaneous transplantable Leydig cell tumour in a Fischer strain rat, reported to secrete steroid, however over a 6 hour period the secretion of oestradiol and testosterone by RIA could not be confirmed.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 43
- Ann Meeting Soc Reprod Abst 1970;4
- J Natl Cancer Inst 1968;41:1141 - PMID: 5688092
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.