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TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, chronic myeloid
DSMZ catalog code: ACC 168
Morphology: single, round to polymorph cells growing in suspension, some cells are loosely adherent
Description: human chronic myeloid leukemia in blast crisis established from the peripheral blood of a 29-year-old woman with chronic myeloid leukemia (CML) (treated 5 years with busulfan from 1979-1984) one month after onset of myeloid-megakaryocytic blast crisis; cells were described to express megakaryocytic and erythroid markers and to respond to induction of differentiation with various reagents; parental cell line of subclone LAMA-87 (DSM ACC 270) confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Prof. D. Seigneurin, University Hospital of Grenoble, Grenoble, France
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.5-1.0 x 106 cells/ml; optimal split ratio of 1:2 to 1:3 every 2-4 days; dislodge any adhering cells by shaking culture flask at 37C with 5% CO2 saturation density at about 2.0 x 106 cells/ml;
doubling time of ca. 50 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (human hypertriploid karyotype with 4% polyploidy; 73(69-77)<3n>) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD11b+ CD13+ CD14- CD15- CD19- CD33+ CD41- CD42b- HLA-DR+ Gly A-) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase negative)
- Exp Hematol 1987;15:822-832 - PMID: 3476310
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.