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TISSUE/ORGAN: mammary gland
ECACC catalog code: 96081411
Morphology: Epi-or myoepithelial
Description: Rat mammary carcinoma
Viruses: See Properties
Depositor: Dr U K Ehmann, Pathology Research 151B, Veterans Affairs Medical Center, Palo Alto, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Connexin 43, Z01 tight junction protein, Transforming Growth Factor (TGF) , int-2, Basic fibroblast Growth Factor (bFGF) Applications: Feeder layer for mouse and rat mammary and mouse thymic cells
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM:F12 (1:1) + 2mM Glutamine + 50ng/ml Hydrocortisone + 5µg/ml Insulin + 5-10% New Born Calf Serum (NBCS). Split confluent cultures 1:10 to 1:20 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C; subculture twice weekly.
Karyotype: Polyploid 80-90 chromosomes, 8H mode
The rat mammary tumour cell line LA7 is a clonal derivative of Rama 25. The parent line was established from a dimethylbenz(a)anthracene- induced mammary cancer in Sprague-Dawley rats. LA7 produces spontaneous ridges in passages up to approximately 25. They have been described as intermediate cell type (F-precursor) between Rama 25 and fusiform cells. Used as lethally irradiated (6000 rads) feeder layer LA7 promote the proliferation and colony formation of primary and sub-cultured mouse mammary, mouse thymus and rat mammary cells. The cell line can be used as a feeder layer up to passage 80 at least.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- In Vitro Cell Dev Biol 1986;22:738 - PMID: 3782011
- In Vitro Cell Dev Biol 1991;27A:749 - PMID: 1717432
- J Cell Biol 1984;98:1026 - PMID: 6699079
- Proc Natl Acad Sci U S A 1979;76:1256 - PMID: 375233
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.