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SPECIES: mouse, C3H/An
TISSUE/ORGAN: areolar and adipose
ECACC catalog code: 85011425
Susceptibility to viruses: bluetongue virus * herpes simplex * pseudorabies * vesic. stomatitis (Indiana)
Description: Mouse C3H/An connective tissue
Viruses: See Properties
Depositor: Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split semi-confluent cultures seeding at 5x1,000 to 2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Subclone of parental strain L, established by W R Earle in 1940. One of the first to be established in continuous culture. The L strain was derived from normal subcutaneous 1 and adipose tissue of 100 day old male C3H/An mouse. Cells are APRT+ and HPRT+.
Research council deposit: No;
Price Code: A;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ICLC: ATL95001
- J Clin Microbiol 1988;26:2324 - PMID: 2853175
- J Natl Cancer Inst 1943;4:165-212
- J Natl Cancer Inst 1948;9:229
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.