This is the complete description of the cell line that you selected.
By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).
TISSUE/ORGAN: skeletal muscle
ECACC catalog code: 92121114
Description: Rat skeletal muscle myoblast
Viruses: See Properties
Depositor: Dr M Shaw, ICI Pharmaceuticals Ltd, UK
No restrictions. Patent: None Specified By Depositor
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split semi-confluent cultures to a seeding density of 1.5-2x1,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C. Cells fuse on reaching confluence, producing myotubes. To prevent fusion maintain the cultures sub-confluent and reclone every 6-8 weeks.
A subclone of L6, a rat thigh muscle cell line. On reaching confluence the cells will fuse to form myotubes and 'twitching' movement will be seen. Once cultures have fused they cannot be recloned.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
- Dev Biol 1970;23:1 - PMID: 5481965
- Proc Natl Acad Sci U S A 1968;61:477 - PMID: 5245982
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.