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TISSUE/ORGAN: peripheral blood
ECACC catalog code: 91012320
Endogenous viruses: contains porcine retrovirus
Description: Porcine peripheral blood T cell
Viruses: See Properties
Depositor: Dr B Kaeffer, INRA, Laboratoire Pathologie, Nouzilly, FRANCE (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: RT assay and virus studies: Porcine retrovirus
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 0.05mM 2-Mercaptoethanol (2ME) + 10% Fetal Bovine Serum (FBS). Maintain cultures between 100,000 to 1,000,000 cells/ml; 5% CO2; 37C. Cells grow as small clumps.
Karyotype: Modal No 36
One of a series established from mononuclear cells of boar (Sus scrofa domestica L) which was injected with Shimozuma cells producing porcine retrovirus (Tsukuba-1). The cells express MSA4. The cells contain retrovirus particles but are negative in RT assay.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Int J Cancer 1990;46:481 - PMID: 2394515
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.