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SPECIES: mouse, BALB/c
TISSUE/ORGAN: bone marrow, mast cells
DSMZ catalog code: ACC 114
Morphology: round cells growing in suspension; during exponential growth cells aggregate in small clusters
Description: mouse mast cell established from bone marrow mast cells of a female BALB/c mouse in 1984; cells are IL-3 dependent and secrete IL-6 confirmed as mouse with IEF of AST, LDH, MDH, NP
Viruses: ELISA: reverse transcriptase negative
Depositor: Dr. L. Hueltner, GSF, Institute for Experimental Hematology, Munich, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80-90% RPMI 1640 + 10-20% FBS + 20-50 U/ml IL-3 or 10% vol conditioned medium of WEHI-3B cell line (DSM ACC 26) cells are initially (after thawing) difficult to grow! after thawing seed at different concentrations in 24-well plates, later maintain at 0.1-1.0 x 106 cells/ml, do not exceed 1 x 106 cells/ml; split up to 1:10 about 1-2 times per week at 37C with 5% CO2 maximal density at about 2.0 x 106 cells/ml;
frozen with 70% medium, 20% FBS, 10% DMSO at about 7 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
- Exp Hematol 1990;18:873-877 - PMID: 2117548
- Immunology 1989;67:408-413 - PMID: 2788129
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.