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SPECIES: mouse, DBA/2
TUMOR: leukemia, lymphocytic
ECACC catalog code: 87092804
Description: Mouse DBA/2 lymphocytic leukaemia
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Cytotoxicity studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Fischer's + 2mM Glutamine + 10% Horse Serum (HS) or ATCC CRCM30 + 10% Horse Serum (HS). Maintain cultures between 5x10,000 to 8x100,000 cells/ml; 5% CO2; 37C.
Karyotype: 2n = 40, diploid
Established from a tumour induced in an 8 month old female 234 sub-line 212 of a DBA mouse. Originally sub-cultured in DBA/2 mice it was adapted to suspension culture. The line has been extensively used in cytotoxicity assays. Develops massive tumours in nude mice after a latent period of 7-10 days.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 219, ICLC: ATL99002
- J Natl Cancer Inst 1949;10:179-192
- J Natl Cancer Inst 1966;36:405-421
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.