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TUMOR: lymphoma, Hodgkin
DSMZ catalog code: ACC 197
Morphology: single cells in suspension or as clusters; polymorph cells, some cells have 'spikes', 1-5% giant multinucleated cells
Description: human Hodgkin's lymphoma established from the pleural effusion of a 37-year-old woman with Hodgkin's lymphoma (stage IVB, nodular sclerosis, refractory, terminal) in 1978 confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. J. Minowada, Fujisaki Cell Center, Okayama, Japan
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.2-0.5 x 106 cells/ml; split culture 1:2 every 2-3 days; after an initial slow start cells grow quickly; seed out at ca. 0.5 x 106 cells/ml at 37C with 5% CO2 saturation density at about 0.5-1.0 x 10 6 cells/ml;
doubling time of ca. 35 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (human flat-moded hypertetraploid karyotype with 10% polyploidy; 94(75-99)<4n>XXXX, +2, -5, +6, -9, -9, +12, -13, +17, +4mar, +2r/dmin, del(1)(q13), der(2)t(2;4)(p14;q21)x2-3, add(5)(p11), add(6)(q24/25)x2, add(7)(q35)x2, del(7)(p 4),) * cytogenetics 2 (add(9)(p22), del(11)(q22.3), del(12)(q21)x1-2, add(13)(p11)x2, add(14)(q32)x2, der(16)t(16;?)(q24;?)t(1;?)(q11;?), del(17)(p12), del(21)(q21.2)x2) * DNA fingerprinting (unique DNA profile with (gtg)5 multilocus probe) * immunological analysis (CD2- CD3- CD13- CD14- CD15- CD19- CD21- CD25- CD30+ HLA-DR+) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase not detected)
- Blut 1979;38:185-190 - PMID: 216443
- Int J Cancer 1980;26:723-731 - PMID: 7216541
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.